Materials

Quantity Item Description

5 mL of the following nine juices:

-Martinelli's Apple Juice

-Juicy Juice Apple Juice

-Washington's Natural Apple Juice

-Seneca Apple Juice

-Minute Maid Apple Juice

-Tree Top Apple Juice

-Tree Top Apple Raspberry Juice

-Tree Top Apple Grape Juice

-Tree Top Apple Pear Juice

1 Pair of Scissors

9 HPLC Vials

9 HPLC Vial Lids

9 Disposable Glass Pipettes

1 Pipette Bulb

1 Carousel

1 High Pressure Liquid Chromatograph

9 Straws

1 Automatic Refractometer

Procedures

High Pressure Liquid Chromatograph:

1. Go to the store and buy all juice.

2. Go to the lab and gather all materials.

3. Label all the juice with numbers.

4. Label all the HPLC vials with the corresponding numbers.

5. Open the first juice and use a glass pipette to fill the HPLC vial

about 2/3 full of juice.

6. Get a new glass pipette and repeat step 5 for the remaining eight

juices.

7. Screw the lids on the HPLC vials and put them into the carousel.

8. Put the carousel into the High Pressure Liquid Chromatograph

and push start.

Refractometer:

1. Stick a straw into the juice, clamp thumb over the end of the straw

and pull it out.

2. Bring the straw over the refractometer and take the thumb off the

end of the straw (juice should drip out).

3. Push Run and record the data.

4. Clean off the refractometer with Kimwipes®.

Get a new straw and repeat steps 1-4 for the rest of the juice.